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Mutation Surveyor® software was used for analysis of sequence data with the program set to check 2D (bi-directional) small peaks; the mutation-calling parameters were set to the program default including the overlapping factor and dropping factor.
All cephalograms were taken digitally by the same operator with a Sirona© Orthophos XGPlus cephalometrical device (Sirona© Dental Systems GmbH, Bensheim, Germany) and set to the program C3F with an image field of 24 × 27 cm.
To construct the framework maps, most of the parameters were set to the program default values; three of them are sensitive for restricting the number of markers in computation, they were LIMIT_DISTANCE = 3, LIMIT_ LIKELIHOOD = 3, and UNKNOWN_COUNT = 2. First, pairwise LOD scores and distances for each pair of markers were computed by using the TSP/CONCORD 'pairlods_dists' program.
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