Exact(4)
AGO7 specifically associates with miR390 and cleaves TRANS-ACTING siRNA3 (TAS3) RNA to set the nucleotide frame for tasiRNA production, but the basis of this specific association is unknown [12].
In this study, we set the nucleotide sequences changing in Kimura-2-Parameter gamma model because we found that transitional substitution rates were much higher than transversional ones in the viral evolution, and substitution rates were significantly different among sites.
For each gene of the selected data set, the nucleotide sequence was aligned based on the amino acid sequence using tranalign/EMBOSS package http://emboss.sourceforge.net/.net/
The alignment consisted of 3,170 positions, of which 935 were constant, and 1,542 were parsimony informative; (2) allnuc data set: the nucleotide sequences of the rRNA, tRNA and protein-coding genes of all available complete mt genomes of neogastropods, one Ranellidae, and one Vetigastropoda, were concatenated into a single data set of 14,156 positions.
Similar(56)
To further explore the possibility that virion-strand specific mutation biases are a feature of geminivirus evolution in general, we determined with greater precision than has been previously achieved with much smaller data sets the nucleotide mutation spectra of MSV (a mastrevrius), and TYLCV (a begomovirus).
BLOG computes for each species in the reference set the distinctive nucleotide positions of the DNA Barcode sequences and the logic classification rules in the form of "if-then" that are able to characterize a species in a compact way.
For the total data set, the mean nucleotide diversity was 0.0047 (SD = 0.0034), the mean value of Watterson's estimator was 0.0067 (SD = 0.0046), and the average haplotype diversity was 0.86 (SD = 0.16).
In our data set the first nucleotide of the A-loop (helix 92, position U2526; E. coli U2552) is predicted to be modified in five of the species and the fifth nucleotide in the loop (position C2830; E. coli C2556) is predicted to be modified in two species.
For each data set, the best fitting nucleotide substitution model was tested with a hierarchical likelihood ratio test following the strategy described by Swofford and Sullivan (2003) [29], using a neighbor-joining (NJ) base-tree with LogDet corrected distances.
In the mosquito data set, the fluctuation of nucleotide transitions caused by the sequences excluded from the core set was very small.
Specifically, 27 (respectively 2) ESTs were annotated, choosing as the search set the non-redundant nucleotide collection (respectively the Expressed Sequence Tags collection) at NCBI.
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