Exact(5)
For each primer set, the efficiency of the PCR reaction was measured in duplicate on serial dilutions (factor 10) of the cDNA sample.
For each primer set, the efficiency of the PCR reaction (linear equation: y = slope + intercept) was measured in triplicate on serial dilutions of the same cDNA sample.
Specific primers were purchased from Qiagen (QuantiTech Primer Assays), for each primer set the efficiency was >95% and a single product was seen on melt curve analysis.
For each primer set, the efficiency was derived from a standard curve (20 0.01 ng) generated with cDNA obtained from a pool of myeloma cells.
For each primer set, the efficiency of the PCR reaction (linear equation: y = slope + intercept) was measured in triplicate on serial dilutions of the same cDNA sample (a pool of 10 samples).
Similar(54)
On the contrary, ina social media setting the efficiency is unbounded and it constitutes an objectivefunction to maximize in order to increase the engagement of the user base.
We use the experimental methodology to identify and compare in a laboratory setting the efficiency properties of these two market mechanisms, given a market structure similar to the existing one in continental Europe, i.e. a competitive oligopoly.
In this subsection, we set the time efficiency parameter C=0.3.
For the remaining alleles, we set the amplification efficiency to the minimum value investigated and considered for this latter all values between 0.01 and 1. T1 was computed 100 times for each number of alleles and minimum amplification efficiency and 10,000 sets of reads were simulated for each run.
The high overlap (81.3%) between the C hr. picta cDNA set from Ensembl Pre! and our data set proves the efficiency of our method, especially when the annotation of closely related species is available.
Chefs set the tone for efficiency, sanitation, esprit de corps.
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