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This was achieved through optimised thermal cycling and the development of a generic degenerate primer set targeting the adenovirus hexon gene.
Finally, the primer set targeting the 16S-ITS-23S 16S-ITS-23S 16S-ITS-23Secific as it amplified DNA from many other Enterobacteriaceae species.
However, of those 4 primer sets, only the primer set targeting the tuf gene also amplified DNA from all Shigella strains tested.
Of the 9 PCR primer sets tested, only 3 of the 5 primer sets targeting uidA gene and the primer set targeting tuf gene amplified DNA from all E. coli strains tested.
To reduce false negatives that can be caused by sequence variability, a new universal primer pair was designed against a divergent sequence data set, targeting the open reading frame 4 (heat shock protein 70 homologue gene), and optimised for conventional one-step RT-PCR and one-step SYBR Green real-time RT-PCR assays.
Two primer sets targeting the STM4495 and SEN1392 genes with high specificity were selected for S. Typhimurium and Enteritidis, respectively, and a multiplex PCR method was developed for the identification of these bacteria based on these two primer sets and another primer set targeting the srfC gene specific for Salmonella enterica.
Similar(19)
Set targeted goals.
"We set targets to 2010.
One of these is to set targets.
task_set_exception_ports - Set target task's exception ports.
We do not set targets for sanctions.
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