The use of a primer set targeted for an intron-containing sequence or RNA extract without contaminating DNA has been established to overcome this problem.
During the individual sessions, which incorporated motivational interviewing strategies, girls set targeted behavioral goals aimed at preventing a spectrum of weight-related problems.
One primer and probe set targeted a hypervariable region of the 16S rRNA gene unique to strain RHA1 fcb) and its phylogenetic relatives, and the other set targeted the recombinant 4-chlorobenzoate (4-CBA) degradation operon (fcb) and was strain-specific.
PCR amplification tests showed that the designed primer and probe set targeted aniline-degrading strains such as Acidovorax sp., Gordonia sp., Rhodococcus sp., and Pseudomonas putida, thereby suggesting that the developed method can detect a wide variety of aniline-degrading bacteria.
The identification of germline T gene duplication in familial chordomas [10] prompted us to perform quantitative real-time PCR in our set of sporadic chordomas using one set of primer/probe targeted to T and a reference control primer/probe set targeted to MCM7.
The first set targeted the mcrA gene from the Methanosarcina acetivorans C2A genome (GenBank accession no. AE010299), and the second set targeted the mcrA sequences pooled from environmental samples.
