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In addition, a prediction of putative novel miRNAs was carried out using the whole set of sequencing data.
The primary set of sequencing primers used for each amplicon is listed in a supplemental material (Table S1).
The performance of the CORE database for identifying clinical sequences was compared to that of three publicly available databases, GenBank nr/nt, RDP and HOMD, using a set of sequencing reads that had not been used in creation of the database.
Based on sequence alignments of H5N1 and sequencing results (data not shown) from all the isolates, a set of sequencing primers were designed spanning a region of the HA informative with regards to clade, strain, receptor binding motif, cleavability and glycosylation (Figure 1) and which specifically hybridize to H5N1.
Any number of complex filters can be designed and applied to a set of sequencing reads.
Added to these reads was another set of sequencing on skin biopsy samples described previously [ 29].
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This approach limits the adjustable parameters to a small set of sequence-independent parameters.
The users can generate a phylogenetic tree and structure-based sequence alignment for selected set of sequences.
For finding the relationship between one sequence and a set of sequences, we cannot apply the traditional LCS algorithms immediately.
The set of sequences encompasses a ~ 500 ka interval, each sequence recording a ~ 100-ka orbitally-driven climate-eustatic cycle.
Let be the set of sequences (2.1).
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