They're sitting at the desk next to you, heads down, twitchy fingers trailing set of jelly-looking fruits.
This second set of fruit were chosen as they would be fully responsive to ethylene.
Overall, 37 genes had peak read amounts within cluster A (the set of fruit-induced genes), and 113 genes within cluster B (the set of AZ-induced genes).
A random set of fruiting genotypes and the two parents were used in each of the three years of the study (2011 2013).
RNA-seq analysis was performed on selected samples at maturity and also on an additional set of fruit harvested 231 DAFB (softened on vine).
One set of fruit was put into a 4000-L plastenttent and treated with 1 µL L−1 1-MCP (SmartFresh, AgroFresh Inc., Springhouse, PA, USA) for 24 h using a release and fan system supplied by the manufacturer.
The process was repeated with different sets of fruits in order to obtain two independent biological replicates at each developmental stage.
These data were confirmed by a biochemical analysis of these metabolites, which showed no statistically significant differences between the two sets of fruits (Additional file 1: Table S4), with the exception of general reducing activity, which was higher in the MYC fruits.
To do this we rearrange Equation (1) as (3) and use this to calculate a set of values of G (the set contains 658 values from, roughly, 100 sets of fruit measurements for each of six time periods, i.e. Days 23, 35, 49, 65, 94 and 140 AFB).
Although a preliminary experiment (not presented) showed that 24-h-old fruit had similar water-loss rates to freshly harvested fruits, a new set of six fruits was sampled and prepared every 3h as described above.
