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To assess functional differences between these two strains, ∼5100 genes were deleted in Sigma for comparison with the same set of deletions in S288c (Winzeler et al. 1999; Dowell et al. 2010).
However, TP73 [13] and CHD5 [15], located on 1p36, are not included in this set of deletions.
Analysis of microhomology at the breakpoints for 1000 random sets of deletions (generated by genome shifts) confirmed this apparent over-representation of microhomology for this set of deletions.
Following this re-analysis, the HWE p-value at this locus was 1.0, indicating that the re-analysed set of deletions is in HWE.
This set of deletions, removing genes involved in expression of type I pili, curli, colanic acid, and capsular polysaccharides, was concurrently identified elsewhere (E. coli MG1655Δfim, ΔwcaL wza, ΔcsgC-csgG) [29].
The novel deletions are the whole set of deletions excluding those 'used' by translocations.
Similar(47)
To do this, we focused on the set of deletion-CNV regions in our molecular validation pipeline and examined each deletion's mean standardized log2 ratios.
In order to better the statistical power, improvement in the reference data set of deletion mutants (e.g., preparation of the replicated data sets) would be required.
We further examined the specificity of the ligand binding domain of ApoEr2 in dendritic spine formation, we used a second set of deletion constructs of ApoEr2 with a C-terminal HA tag (Fig 3D).
To identify genes that regulate mitosis in yeast, an SGA screen of the mad2Δ strain as a query mutation was performed against a complete set of deletion-mutant yeast strains [24].
Future studies may warrant a phenotypic-response screening of the entire set of deletion mutants of S. cerevisiae to the benzaldehydes.
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