Exact(2)
Although the annotation process suggests that each of the plasmid classes seems to possess a different set of conjugation genes, the fact that all 11 plasmids are related, sharing large blocks of DNA sequence tends to suggest that the conjugation systems themselves will also be functionally closely related.
Intriguingly, although ubiquitylation was directed at K164 of PCNA, the modification did not involve the set of conjugation factors required for damage-induced ubiquitylation, but instead the Cullin 4-based ubiquitin ligase CRL4, which is known for assembly of K48-linked ubiquitin chains (Lo et al. 2012).
Similar(58)
We firstly introduced by conjugation a set of 17 conjugative ESBL plasmids (CTX-M-1, -2, -9 and TEM-52) of IncHI2 and IncI1 groups in the SGI1-carrying S. Agona strain 959SA97 previously described to conjugally transfer SGI1 in the presence of the conjugative IncA/C helper plasmid pR55 (Table 1 and data not shown) [15].
In conclusion, we have developed a diverse set of site-selective conjugation methods suitable for the synthesis of glyco-CRM197, conjugates with various carbohydrate loading and attachment points.
The plasmid pSMC2 carries a set of genes for conjugation (the tra and trb genes), and although lacking traJ and traT, it can be transferred by conjugation from SM39 to E. coli K-12 strain x1037 Rifr (data not shown).
NEDD8 utilizes a distinct set of enzymes for conjugation to proteins [ 9].
The strain IOEB_C52 contains a contig with genes that are typical of conjugative plasmids: a complete set of the Trs proteins, conjugation proteins, integrases, and transcriptional regulators.
The sex factor of the strain MG1363 contains 59 putative genes [ 6], 10 of which are predicted to encode proteins that are evolutionary conserved between 5 ancestrally related Gram-positive coccal conjugation systems [ 47] and are suggested to be part of a minimal set of genes required for conjugation.
Nevertheless, using the lux-monitored conjugation assay with an F- lux plasmid, the same set of LPS mutants showed strong conjugation inhibition (Fig. 6B) suggesting a severe defect in the recipient ability in liquid mating conditions.
To set up conjugation, cells of different mating types were mixed.
The quadratic inverse eigenvalue problem (QIEP) is to find the three matrices M,C, and K, given a set of numbers, closed under complex conjugations, such that these numbers become the eigenvalues of the quadratic pencil P= λ2M+λC+K.
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