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It might be assumed that for one set of assay conditions, the number of spots counted after varying the number of cells added to a well would be normalized (by calculating the number of spots per million PBMC) to give the same result.
Three replicate cultures without substrate were operated as controls for each set of assay conditions.
In addition, the precise conditions for each set of assay conditions should be given in all figure and table legends.
Since laboratories have developed their in-house IVS techniques independently, a diverse set of assay parameters are in use regarding cell concentration/density, length of culture, peptide concentration and type and number of exogenous cytokines, among others [ 16].
An equivalent set of assay experiments were carried out using the hemicellulose-rich solid extracted from the straw, at reaction temperatures of 180 and 200°C in an aqueous solution of 1% (w/w) sulphuric acid.
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The novel set of assays was evaluated by testing 112 EDTA plasma samples.
This set of assays includes approaches to monitor ARS activity in vitro, in human cells, and in bacteria.
The set of assays lacked automation of DNA purification and of PCR mixture preparation, and was not furnished with measures to monitor for sample adequacy.
It appears that using data from a diverse set of assays and employing a prediction network to select assays for inclusion in the combined model is a powerful approach.
For each set of assays a standard curve using pure ATP (Sigma, St . Louis MO) in serial dilutions was carried out to overcome any experimental variances, operator differences, and instrument discrepancies, etc.
Recently, I reported on a set of assays for several DNA-modifying enzymes (polymerases, endonucleases, and ligase) based on simple, hairpin-type oligonucleotide substrates labeled with a single fluorophore (Anal. Biochem. 412 (2011) 229 236).
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