As improved tools make transgenesis more and more efficient, the stage has been set for precise modification of the zebrafish genome such as are done in other model organisms.
(A ) M data set for 1M7 modification across 188 single mutations along the GIR1 ribozyme sequence.
(A ) M data set for 1M7 modification across 127 single mutations along the class I ligase sequence.
(A ) M data set for 1M7 modification across 168 single mutations along the AdoCbl riboswitch aptamer sequence in the presence of 60 µM AdoCbl ligand.
Fixed modifications were set for carbamidomethylation of cysteine and variable modifications were set for oxidation of methionine.
As variable modifications, oxidation of histidine, tryptophan or methionine, dioxidation of tryptophan and tryptophan to kynurenin modification were allowed, fixed modification was set for carbamidomethylation of cysteine.
Variable modifications were set for methionine oxidation, N-terminal acetylation, and phosphorylation on serine, threonine, and tyrosine residues.
To search the MS/MS data, dynamic modifications were set for oxidized Met (+16) and phosphorylated Ser, Thr, and Tyr (+80).
Variable modifications were set for carbamidomethylation, +57.02 Da; N-terminal acetylation, +42.01 Da; oxidation of methionine, +15.99 Da; and phosphorylation of STY, +79.97 Da.
Variable modifications were set for carbamidomethylation, +57.021 Da; N-terminal acetylation, +42.011 Da; oxidation of methionine, +15.995 Da; and phosphorylation of Ser, Thr or Tyr, +79.966 Da.
