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To determine if nitrogen pools are depleted during our fermentation study, we did a careful analysis of the homozygous deletion set fermentation compared with the EC1118 wine yeast strain by monitoring total YAN along with metabolites, growth, and weight loss measurements.
The P. stipitis cells and respective fermentation media (xylose rich) were fed to the fermentation vessel, after the set fermentation time Z. mobilis cells and respective media were fed to the same vessel.
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When the initial OD of approximately 1.0 was used for setting up fermentation experiments, the medium was supplemented with 0.42 g/l Tween 80 and 0.01 g/l ergosterol.
Five residence times were set after a fermentation condition was modified to reach a new stationary state.
Forty percent air is a typical DO set point in fermentation of many cell types.
Fermentation set points used were 30 +/− 1 °C, dissolved oxygen 30%, air flow 0.5 1.0 SPLM, and agitation 500 rpm.
Samples from two different fermentation conditions were analyzed: one set from a control fermentation with no salt and one set from a treatment fermentation with 0.5M NaCl.
CA produced the strain set and performed fermentations (experiment B).
In fed-batch fermentations set point of substrate (100% methanol or 50% glucose) feed rate changed exponentially [ 33].
Our objective was to establish a promising set of fermentation parameters for high gravity multi-stress fermentation of the hemicellulose rich water soluble fraction from steam treated Douglas-fir while developing a better understanding of the physiological/biochemical basis of yeast inhibitor tolerance.
The agitation speed and pH of seed culture were maintained at 500 rpm and 5.5, respectively, while the agitation speed during fermentation was set as 500 rpm at the first 6.5 h and then controlled at 800 rpm.
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