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(You can even set designated areas on the screen to look for motion, which allows you to exclude, say, wind-blown trees).
This amended ES expression set (designated the ES set without proliferation genes) could also segregate the 101 pretreatment tumor samples according to time to progression.
A non-cross-validated PLS analysis was performed, and the final parameters and statistics (q2 = 0.508, r2 = 0.948) for the common training set (designated Class I+II) are summarized in the first row of Table 1.
The cDNA produced was subjected to real-time qPCR as above, using the primer/probe set designated 'late', and was normalised for input cDNA by primers and probe targeting GAPDH transcripts (300 nM forward primer GAPDH 5'- CCACATCGCTCAGACACCAT-3',300 nM reverse primer GAPDH 5'-CCAGGCGCCCAATACG-3', 150 nM probe GAPDH 5'-6-FAM-AGGTGAAGGTCGGAGTCAACGGATTTG-BHQ1-3').
For the first kx/km set (designated as set u), kx and km were 1.0 and 0.0, respectively.
The first data set, designated ER232, contained 232 chemicals, 131 active, and 101 inactive that were tested in our lab (Fang et al. 2003a).
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Probe sets designated by Affymetrix not to cross-hybridize outside of their gene family were selected if they had a consensus sequence > 250 bp and had a BLASTx match with an E-value < e-30.
For genes that were selected from chromosomal regions of interest and not necessarily on the basis of differential expression, correlations were carried out using data from the probe set deemed most specific for the gene of interest by the Affymetrix software (e.g. microarray probe sets designated -at are considered more specific than -s-at and -x-at probe sets).
"Up-regulated gene sets" and "Down-regulated gene sets" designate GSEA gene sets overexpressed or underexpressed, respectively, in NF1-affected individuals or Nf1+/- mice as compared to NF1-unaffecteds or wild-type mice.
Setting: Designated quaternary service for GUC in a tertiary referral centre.
(By the way, set designer Anna Louizos designates the 50th state by putting an "Aloha" sign above an airport gate, along with a lanai or two elsewhere).
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