The results showed that unlike the ten miRNA signature, the four miRNA did not consistently correlate with patient survival in training and testing set (data not shown).
This approach resulted in the identification of 54 genes distinguishing accurately between the two classes of samples, as demonstrated by analysis with the testing set (data not shown; Table 2).
Amplification of strand cDNA was undetectable with the strand primer set, and conversely amplification of strand cDNA was undetectable with the strand primer set (data not shown), confirming a high level of fidelity for the intended target strand.
In contrast, GSEA analysis of the Δ gcn5A mutant parasites showed no significant enrichment for any oocyst gene set (data not shown).
In addition, several metal ion resistance genes and stress-related proteins also belong to the variable gene set (data not shown).
Sensitivity analysis also found that no single study can substantially change the pooled ORs of other data set (data not shown).
The data for the orthologous AS gene set was found to be very similar to that of the complete AS gene set (data not shown).
Doing so changed the compositions of the 20-case early death and long survival groups with the consequence that immune system genes were less prevalent in the most predictive gene set (data not shown).
These primers significantly advanced the rate of ERCA amplification, cutting the reaction time in half for the Factor V Leiden probe set (data not shown), resulting in a 20-minute assay.
Similar results are obtained when searching against either the JGI filtered gene set or DSM gene set (data not shown) suggesting there is no bias produced by a given gene set.
Sensitivity was measured on all metazoan miRNA hairpins (3,902 hairpins) instead of on C. elegans miRNA hairpins (132 hairpins) because of the inaccuracy caused by the limited number of miRNAs in the C. elegans set (data not shown).
