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The cells were exposed to compounds at set concentrations for 120 h: PRI-2191 and PRI-2205 100 nM; 5-FU 0.1 μg/ml.
The cells were exposed to compounds at set concentrations for 48 h: PRI-2191 and PRI-2205 100 nM; 5-FU 50 and 200 μg/ml.
We converted absorbance to (Ceq) through a calibration curve (Supporting Information, Figure S4B) calculated from the linear regression of absorbance measurements at 570 nm of anhydrous APTES solutions with ninhydrin at set concentrations.
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However, the solid nanosuspension stabilized by surfactants showed the worst formability potential when be applied in setted concentrations during freezing and lyophilization.
(A ) Recombinant PERK cytoplasmic domain was incubated at a set concentration of IPA.
A set concentration of glucose (11.1 mM) in RPMI 1640 media (Gibco, USA) was used as a control.
The atmospheric CO2 concentration of the air within each chamber was controlled by adding either CO2 or CO2-free air to maintain the set concentration.
In typical experiments, various amounts of TRAP protein were titrated against a set concentration of the labelled RNA, in the presence or absence of L-Trp (L-tryptophan).
By varying each standard across the full range of copy numbers, some of the confounding influence caused by individual microarray probe specificity, which is present when a single spike-in is used at a set concentration, is negated [ 20].
The stirring speed was controlled by dissolved oxygen (DO) concentration set at 15%.
To simulate these perturbations, we set the concentrations of Bcd or Hb to zero without further adjustment of the coefficients.
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