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Helium was the carrier gas and was set at a flow rate of 1.12 mL/min.
Nitrogen sheath gas was set at a flow rate of 30 L/min.
The carrier gas is hydrogen, and it was set at a flow rate of 2 ml/min.
Nitrogen auxiliary gas was set at a flow rate of 10 L/min and the spray voltage was set to 4.5 kV.
Oocytes (n = 535) were recovered by means of an aspiration pump (set at a flow rate of 10 mL H2O/min), with a disposable 20 G needle attached.
A separate GC injection port (Perkin-Elmer Autosystem XL) was used to load known volumes (1 5 μL) of the calibration standard onto a Tenax TA sampling tube using helium as a carrier gas set at a flow of approx 1 cm3 s-1.
Similar(43)
The carrier gas was helium, set at a constant flow of 1 mL/min through the purge flow rate of 20 ml/min and an equilibrant time of 1 min.
The flow rate of Ar sweep gas was set at a constant flow rate of 100 mL/min using a metering valve and a calibrated rotameter.
The flow rate of the carrier gas, helium, was set at a constant flow rate of 1.4 mL/min.
The oven temperature was set a 200°C and the carrier gas (helium) was set at a total flow rate of 1 ml/min.
Helium set at a constant flow rate of 1 ml/min was the carrier gas.
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