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Single oocytes were washed 3 times and set aside for processing and the remaining cells, considered "oocyte-free" granulosa cells, were carefully pipetted to a different Petri dish with M2, washed 3 times, and set aside for processing.
For "complete oocyte" samples, the oocytes with granulosa were briefly washed in M2 and mouth-pipetted to a different Petri dish with M2 and set aside for processing.
Similar(58)
To assess the quality of the cDNA, samples were screened by PCR with the primers oTM085 and oTM086 specific to the N. benthamiana actin gene (AY179605) and only those samples producing the expected 0.36 kb fragment were set aside for further processing (data not shown).
Set aside for 20 minutes.
The part set aside for Rose presumably?
Set aside for 1/2 hour.
Set aside for 15 minutes.
Also in the building are a scholars' reading room, with cubicles for six researchers; a processing room where materials will be examined and catalogued; and several offices, including the one that had been set aside for the cardinal.
Set aside for about 15 minutes.
set aside for their derivatives contracts.
Predicted insertions and deletions were set aside for assay development.
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