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Of the eight pairs of SCAR primers, seven resulted in the amplification of single bands of the original RAPDs, and the remaining primer set amplified an additional band.
The primer set amplified a specific 1193-bp DNA fragment from F. columnare strains but not from related bacteria, F. psychrophilum, F. aquatile, F. branchiophilum, or other bacterial pathogens of fish, Flexibacter maritimus, Cytophaga johnsonae, Edwardsiella ictaluri, E. tarda, Aeromonas hydrophila, and Streptococcus iniae or from the non-fish pathogen Escherichia coli.
The Hes6 primer set amplified a 252 base-pair DNA segment of the Hes6 cDNA.
The EG95 primer set amplified a single fragment ranging between 1,263 (E. oligarthrus) and 1,316 bp (E. vogeli).
Neither primer set amplified sequences similar to basidiomycetes, even though ITS and SSU sequences from the group 4 isolate (HM596870 and HM195295) clearly align with basidiomycetes, and the ITS sequence was placed with the basidiomycete outgroup in Figure 1.
In 5/15 tested cases (including CH34 which had intact PTEN expression), equivocal PTEN methylation specific PCR results were obtained in which only the MSP2 primer set amplified the methylated target at nucleotide −298 upstream in the promoter sequence while the MSP1 primer set targeting nucleotide −984 did not.
Similar(43)
This primer set amplifies the whole ORF and a part of the 3'UTR.
If JC7-1 is present at the insertion site, the first primer set amplifies a 242 bp fragment of the upstream flanking sequence of JC7-1, and the second primer set amplifies 182 bp downstream fragment of the flanking sequence of this retrotransposon marker.
Briefly, this multiplex primer set amplifies a small fragment of 89 base pairs from the endogenous plasmid and a fragment of 160/157 base pairs from the Variable Region 2 of the ompA gene.
The 24 primer sets amplified a total of 25 loci, with the primers for One102 amplifying two loci with nonoverlapping size ranges (designated One102a and One102b).
Of 27 primer sets tested, 17 primer sets amplified more than one target.
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