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Each case was represented by two tissue cores, 1.5 mm in diameter, with each TMA block also containing cores from various neoplastic, non-neoplastic and reactive tissues serving as assay controls.
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Tubes containing supplemented medium but no tissue were incubated, sampled, and analyzed along with experimental samples to serve as assay blanks.
The use of core streptavidin as scaffold for the display of recombinant anti-immunocomplex peptides will remove these limitations, because they can be combined with the broad options of biotinylated reagents that could serve as assay tracers.
Volume of 200 μl TS broth was used as assay sterility control in all wells of row 11 while 10 μl bacteria culture in 190 μl TS broth served as the assay growth control in all wells of row 12. Plates were incubated in a shaker incubator (Stuart, UK) at 120 rpm, 37°C for 24 hours.
A goat antihuman IgG antibody was obtained from Zymed Laboratories, Inc. (Thermo Fisher Scientific) and served as the assay PC.
Although no future president served as an assay commissioner, Comptroller of the Currency Charles G. Dawes was a commissioner in 1899 and 1900; he was Vice President of the United States from 1925 to 1929.
Seawater served as the assay buffer.
Goat anti-mouse antibody coated wells served as negative assay controls.
BW284c51 served as an assay control.
Doubly cross-linked DnaN ('XX-DnaN') serves as internal assay control as visualized by immunoblotting.
'XX-DnaN' serves as internal assay control visualized by immunoblotting of cross-linked species of DnaN protein (bottom panel).
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