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This was combined with an unusually warm,clear summer and smoke from wildfires in the US that served to stain the ice and make it less reflective.
DAPI served to stain the nuclei.
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The blue fluorescence resulting from the Hoechst 33258 (Sigma-Aldrich, St . Louis MO, USA) staining served to label the nuclei, the red fluorescence resulted from the rhodamine-phalloidin staining served to label the cytoskeleton, and the green fluorescence represented the FITC-MMC.
Propidium iodide (PI) staining served to exclude analyses of dead, potentially autofluorescent cells visualized by standard rhodamine filters.
Gram stain served to identify Gram positive cocci in 94 (22%) samples.
The HE stained sections served to make sure that only the desired tissue type had been harvested; this staining was done using routine methods.
Therefore, Draq5™ staining additionally served to examine the status of nuclear DNA and to identify morphological alterations of the nuclei, such as those that are typical for cells undergoing programmed cell death.
Isolectin B4 and laminin staining served to highlight a significant decrease in the density of fetal vessels with a simplified vascular branching pattern and dilated maternal spaces.
In addition, vimentin and pan-cytokeratin staining served to differentiate between cell types, corroborating our morphological observations, which imply that the presence of tumor-associated fibroblasts is a fundamental requirement for the growth of tumor cells.
All sections showed a faint background staining, which served to count the whole cell number.
Staining of β-actin served to control for equal amounts of protein used in each lane.
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