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The percentage of correct responses over the first 3 days of each phase served as readout of initial learning capabilities in the 5CSRTT training.
Induction of HO-1, an enzyme decomposing heme to biliverdin, iron, and CO2 and accounting for many beneficial outcomes on Nrf2 activation (e.g., [ 22- 24]), served as readout for Nrf2 activity.
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LC3-II present on the internal membrane of the autophagosome is also degraded, so that its consumption serves as readout of the autophagy flux [ 21].
While fractin and cleaved tubulin staining can serve as readouts of caspase-3 versus caspase-6 activity, one must keep in mind the possible limitations of these markers.
The lateral diffusion of the QD-tagged GPI was determined by single particle tracking (SPT) and served as a readout for the plasma membrane (Fig. 5A, Movie S2).
Co-staining for Cora served as a readout of SJ integrity.
Concomitantly, BBR reduced the mRNA expression of Gli1 (Fig. 1c), a transcriptional target of Gli, which served as a readout of Gli activity.
Brdu assay revealed that BBR dose-dependently inhibited the growth of medulloblastoma cell growth (Fig. 4a), concomitantly with comparable reduction of the mRNA expression of Gli1, and ptch1 (Fig. 4b, c), which served as a readout of the Hh pathway activity.
We also examined pCDK which served as a biomarker readout for loss of Wee1 activity in these cells.
Analogous to the two-hybrid system, the libration of the reporter serves as a readout indicating the reconstruction of ubiquitin.
The LH content of the outgoing medium serves as a readout of the GnRH signaling pathway activation in the cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com