The supernatant was vacuum-dried and dissolved in 1 mL methanol and served as extracts for the analysis of TPC, TFC, free radical scavenging activity, and secondary metabolite analysis using HPLC.
A bacterial cell suspension (prepared in the appropriate broth) of 100 μl, corresponding to 1 × 106 CFU/ml, was added in all wells except those in columns 10, 11 and 12, which served as saline, extract and media sterility controls, respectively.
The exiting streams will serve as extracting solvent or scrubbing agent solution in other separation units, by which the overall chemical consumptions of an entire process can be decreased.
Embryonic human brain extract served as positive control since it is known to express high level of PSA-NCAM, as do medulloblastoma (MB) tissues [ 16], which was confirmed in the current study by immunohistochemistry and ELISA for MB (150-1030 PSA-NCAM/μg/μg of protein, n = 3) and embryonic human brain (1600 pg PSA-NCAM/μg of protein).
Buffered minimal methanol yeast extract (BMMY) medium served as induction medium that contained yeast extract (1% w/v), peptone (2% w/v), yeast nitrogen base (1.34% w/v), biotin (4 × 10−5% w/v), methanol (0.5% v/v), potassium phosphate buffer (100 mM, pH 6.0), and glycerol (1% v/v) replaced with methanol (0.5% v/v).
Egg masses placed in distilled water without crude antibiotic extract served as control.
Non-illuminated and illuminated tubes without enzyme extract served as control.
In this present work, we synthesized zero-valent iron nanoparticles (ZVIN) using reproducible Calotropis gigantea (CG) flower extract served as both reducing and stabilizing agent by completely green approach.
All reactions were incubated for 24 h, followed by treatment with 20 µg proteinase K at 65°C for 10 min. Same reaction performed with heat inactivated (65°C for 10 min) extract served as negative control (called "Input") in all experiments.
TNT HIF-1α-myc substrate in the absence of reaction solution or cell extract served as a negative control for the unmodified electrophoretic mobility of the HIF-1α-myc protein.
