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H3 served as a loading control.
GAPDH was served as a loading control.
Syntaxin 1B levels were only slightly increased in CHL1−/− lysosomes, while levels of the cell adhesion molecule L1, which served as a loading control, were similar in both genotypes (Fig. 9B).
Results from Western blotting experiments are depicted in Figure 3. Notably, FOXA2 siRNA Probe 3 caused a significant reduction in FOXA2 and a minor reduction in HNF4alpha expression while actin served as a loading control.
Ambion Bright-Star membranes pre-hybridized in ULTRAhyb (Ambion) for 30 minutes at 68°C, and probed overnight with 32P-labeled dfmr1 fragment or 32P-labeled β-tubulin fragment, which served as a loading control.
Calnexin served as a loading control.
ef1α served as a loading control.
5S rRNA served as a loading control.
α-tubulin served as a loading control.
Oct-1 served as a loading control.
tRNAThr served as a loading control.
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