While fractin and cleaved tubulin staining can serve as readouts of caspase-3 versus caspase-6 activity, one must keep in mind the possible limitations of these markers.
The percentage of correct responses over the first 3 days of each phase served as readout of initial learning capabilities in the 5CSRTT training.
LC3-II present on the internal membrane of the autophagosome is also degraded, so that its consumption serves as readout of the autophagy flux [ 21].
Induction of HO-1, an enzyme decomposing heme to biliverdin, iron, and CO2 and accounting for many beneficial outcomes on Nrf2 activation (e.g., [ 22- 24]), served as readout for Nrf2 activity.
Measuring GFP intensity would serve as a readout for the transcriptional activity of the NANOG promoter.
This has complicated efficient drug development, as response rate is less likely to serve as a readout of efficacy, at least when ipilimumab is used as a monotherapy.
The burst content could then serve as a readout of the previous cortical state, which could provide useful clinical information when monitoring patients during burst suppression.
While measured actomyosin density gradients serve as a readout of cortical contractility, cortical tension in general is related not only to contractility but also to viscous forces arising from flow (Mayer et al., 2010) and flow-induced filament alignment (Salbreux et al., 2009).
The skeletal elements exhibit characteristic morphologies and organization that serve as convenient readouts for regulation of morphogenesis.
Correct splicing and enhanced green fluorescence protein (EGFP) upregulation serve as a positive readout for peptide-PMO (PPMO) entry into cells and access to EGFP-654 pre-mRNA in the nucleus.
This suggests that phosphorylation of S6K or its downstream target rpS6 may not serve as an accurate readout for inhibition of all mTORC1 kinase activity.
