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HRP (2 mg/ml in DMEM containing 1% serum) was administered with or without various amounts (200, 500 and 1000 MOI) of wild type baculovirus on HepG2 cells.
An anti-rabbit HRP linked antibody in blocking serum was administered for 1 h.
Upon removal of blocking solution, primary antibody, diluted in 2% goat serum, was administered for one hour.
In passive immunization experiments, 35 μl of heat-inactivated serum was administered intranasally under isoflurane sedation 24 h before and 24 h after HRSV challenge.
One day before and 1 day after HRSV challenge, 35 μl of heat-inactivated SHe-KLH or KLH mouse immune serum was administered intranasally to mice sedated with isoflurane.
Because the intranasal route of serum administration might affect the mechanism by which SHe-specific antibodies hamper HRSV replication in the lungs, the role of alveolar macrophages was also investigated when SHe immune serum was administered parentally.
Similar(54)
To investigate the dose effect of passive immunization with SHe immune serum, different amounts ranging from 0 to 50 μl of SHe-KLH serum were administered intranasally to mice 1 day before and 1 day after HRSV challenge.
For in vivo neutralization during JHMV infection, 0.5 ml of anti-CXCR2 or control normal rabbit serum (NRS) was administered intraperitoneally (i.p). on days 12, 14, 16, 18, and 20 p.i.
In a pig model, bovine serum albumin was administered to induce arthritis in bilateral knees [ 82].
In patients in control group (Group C) equal volume of serum physiologic was administered as a bolus and infusion via epidural catheter during the operation.
3 5 In the present study, purified rabbit serum PON1 was administered to rats intravenously and concentration of dichlorvos in blood was detected at different time points to analyse the effect of PON1 on toxicokinetics of dichlorvos.
Related(20)
blood was administered
awareness was administered
sample was administered
sampling was administered
saline was administered
vaccine was administered
testing was administered
drug was administered
con was administered
antibody was administered
cell was administered
plasma was administered
serum was purchased
serum was determined
serum was taken
serum was received
serum was diluted
serum was moved
serum was collected
serum was used
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