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For detection of antibodies the Pourquier® ELISA Chlamydophila abortus serum verification kit (Montpellier, France) was applied.
abortus among the bulls was investigated with the commercial Pourquier® ELISA Cp. abortus serum verification kit.
The Pourquier ELISA Chlamydophila abortus serum verification test (Institut Pourquier) uses a recombinant fragment of an 80 90 kDa polymorphic outer membrane protein as antigen.
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Our device retained the capability of gender verification when used in serum and serum stains.
The Department of Medical Biochemistry performed a small verification analysis, using serum and citrate plasma from 21 healthy volunteers (data not published).
We then performed multiplexed verification of the in serum unknown detectability of these candidate biomarkers by analyzing enriched serum samples of five MPM subjects (Additional file 5: Skyline file).
Gender verification of real samples (39 serum samples) demonstrated excellent sensitivity and specificity of this smartphone based device.
Open image in new window Fig. 6 WB verification of SEPs in mouse serum.
One limitation of our study is that we did not have a verification of zonulin using the human serum samples after administration of LP.
We confirmed this upon preliminary verification using an ELISA and found that serum levels of Vitamin D-binding protein showed little ability to discriminate patients with ovarian cancer from healthy controls (data not shown).
In this study, we employed Solexa sequencing of pooled sera samples followed by multiple RT-qPCR verification assays of individual samples to generate disease-associated serum miRNA profiles.
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