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The protocol includes repeated serum sampling.
At time of diagnosis blood samples were taken from 79 patients, after 3 months 35 patients were available for a second serum sampling and after 1 year there were 10 patients for a third serum sampling.
However, only three of these 19 women had serum sampling less than 5 years prior to diagnosis.
The Wilcoxon signed-rank test was also used to compare CXCL10 and CXCL13 levels in patients who underwent follow-up serum sampling.
At the time of prostatectomy and serum sampling all patients included in this study were free of clinically detectable bone lesions or other distant metastasis.
Serum samples were collected from prolonged critically ill patients (median ICU stay at day of serum sampling was 11 days) and healthy volunteers after informed consent.
This study included serum sampling and HIV testing.
The time interval between serum sampling in the hypertensive women is also shown in Table 1.
Serum sampling was performed in parallel.
Healthy volunteers were enrolled as negative controls for serum sampling.
For each individual patient, serum sampling and disease activity evaluation were done at the same time.
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