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Serum samples were treated per manufacturer's instruction with receptor-destroying enzyme (RDE) before testing.
Therefore, the serum samples were treated with RDE and retested again.
As control, serum samples were treated with a Ni2+-resin Ni2+-resinh mock-transfected celoadedracts.
Briefly, serum samples were treated with receptor-destroying enzyme (RDE, cholera filtrate) at 37°C for 18 hours, and then heated at 56°C for 30 minutes.
The serum samples were treated with receptor destroying enzyme (RDE) from Vibrio cholerae (Denka Seiken, Cat#370013) for 18 h at 37°C and then were heat-inactivated at 56°C for 30 min according to WHO's standard procedure.
Prior to testing by the HI assay, serum samples were treated with a 1∶5 (vol/vol) of receptor destroying enzyme (RDE, prepared by CNIC) at 37°C for 18 hours followed by incubation at 56°C for 30 minutes.
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In FTA-ABS a patient's serum sample is treated to remove nonspecific antibodies and then is applied to a slide that has T. pallidum antigens on its surface.
A volume of 100 μl of each serum sample was treated at 4°C with SwellGel Blue Albumin Removal Kit (Pierce, Rockford, IL, USA) according to the manufacturer's instructions.
The assays were performed at room temperature and both undiluted serum and urine samples were treated in a similar way.
The control samples were treated with the preimmune serum.
To detect Paneth cells, samples were treated with 10% goat serum (Zymed) for 30 minutes followed by exposing samples to anti-lysozyme (Dako) antibody at 4°C overnight.
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