Sentence examples for serum samples were stained from inspiring English sources

Serum samples were stained for imaging as follows: 6 μL of serum was mixed with 10 μL of NBD C6-ceramide (1 mg/mL in DMSO) and diluted to 1300 μL using an aqueous solution of the density-forming solute (NaBiEDTA) followed by incubation for 30 min to achieve saturation.

After being blocked with 10% goat serum in PBT, the samples were stained with primary antibodies.

Prior to staining, samples were blocked with 5% fetal bovine serum and FcR reagent for 20 min. Samples were stained with CD133-PE and CD34-FITC antibodies (Miltenyi Biotech, Germany).

The samples were stained in the blocking buffer (0.1% TX-100 and 10% goat serum or donkey serum in PBS) containing primary antibodies overnight at 4°C.

Human PDAC and breast cancer tissue microarray (TMA) samples were stained using anti-GCSF IgGs (ab9691, Abcam) diluted 1 25 in blocking buffer (5% goat serum, 2.5% BSA in 1× PBS) on Bond Rxm autostainer.

For immunostaining, samples were stained with Alexa Fluor secondary antibodies.

The samples were stained with calcofluor white.

These samples were stained by IF.

Samples were stained as indicated.

All serum samples were analyzed for the presence of AQP4-IgG and- IgM by an extracellular live cell staining IF technique as previously described [24], [25], [26].

Serum samples were collected from 2003 through 2007.