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All serum samples were split into 3 aliquots and stored at icddr,b at −70°C.
The protocol was adapted according to Müller HM. et al. [ 25]. Isolation was performed according to the manufacturer's instruction except the following protocol steps: the 800 μl of serum samples were split into 2 aliquots of 400 μl and each mixed with 400 μl of Roche Binding Buffer and 80 μl ProteinaseK (20 mg/μl Fermentas, St.Leon-Rot, Austria).
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Purified nucleic acid from a serum sample was split into two aliquots (DNase I-treated and untreated).
To capture the unconjugated forms, the serum sample was split into two aliquots, one to measure the total concentration of each of the 15 metabolites (that is, the sum of conjugated plus unconjugated forms); the other, to measure the unconjugated forms.
The samples were split into two aliquots.
Subsequently, the samples were split in two.
Surgical samples were split in two fragments.
Samples were split into two 5 L samples for analysis.
Skins samples were split into two technical replicates.
Then, the samples were split into two fractions.
Briefly, ambient water samples from each sample were split in two, a treatment and control.
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