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Serum samples were prepared and submitted to following measurements: 25-hydroxycholecalciferol (25D), calcium, phosphorus, magnesium, glucose, triglycerides and parathyroid hormone (PTH).
In brief, pooled serum samples were prepared from tumor and control groups by combining equal volumes of serum from each mouse within the same group followed by thorough mixing.
Their plasma and serum samples were prepared.
Briefly, serum samples were prepared and deproteinized by heat denaturation.
The serum samples were prepared using liquid-liquid extraction technique.
The animals were bled through the tail vein weekly, and serum samples were prepared.
Similar(30)
Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent.
A serial dilution of serum samples was prepared (log4).
All serum samples were first heat-inactivated at 56°C for 30 minutes; then, 2-fold dilutions of each serum sample were prepared in 96-well plates to obtain dilutions of 1 4 to 1 256.
A set of serum control samples were prepared by combining batches of male and female serum (blood bank of Aarhus Sygehus, DK), respectively, and distributed into 3.6 ml portions and stored at -80°C.
For Western blot, serum protein samples were prepared in Sample buffer with EDTA-free protease and phosphatase inhibitors (Thermo, USA), heated at 95 °C for 5 min, ran on a 10% Tricine-gels and transferred to 0.4 μm PVDF membranes (Merck, Germany) at 100 mA for 30 min.
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