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Two 300 μL serum samples were made up to 550 μL with phosphate buffer in D2O and transferred to 5 mm NMR tubes for direct analysis.
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Duplicate injections of each sample were made.
In field-screening laboratories, animals were sedated, serum samples were collected, and veterinary evaluations were made.
CSF specimens were obtained from the patients by lumbar puncture on the same day serum samples were obtained, when neurologists and rheumatologists made the diagnosis of NPSLE in each institution.
Appropriate positive- and negative-control serum samples were tested on each slide together with twofold dilutions of patients' serum samples made in 3% nonfat dry milk in phosphate-buffered saline (PBS).
To confirm the assignments made from 1H-NMR and T2-edited spectra, some blood serum samples were also examined using 2D [1HSQCC] HSQC.
Serum samples were pre-diluted 1 20 and whey samples 1 2.
Upon blood processing and serum recovery, serum samples were stored at −80 °C until further use.
For NMR analysis, serum samples were thawed on ice.
Serum samples were stored at −20 °C until use.
Serum samples were tested anonymously to maintain confidentiality.
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CEO of Professional Science Editing for Scientists @ prosciediting.com