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To avoid uncontrolled degradation of serum components arising from intact proteases and peptidases, all serum samples were immediately heated at 100 °C for 10 min after only one freeze-thaw procedure.
Serum samples were immediately stored at -80°C until analysis.
Serum samples were immediately centrifuged and stored at -80°C until analysis.
Their serum samples were immediately stored at -20°C until analysis.
Serum samples were immediately frozen at −80 °C until the performance of the experiments.
Whole blood and serum samples were immediately frozen and kept at -80°C until analysis.
Similar(47)
Also, a serum sample was immediately frozen (-70°C) and stored for further assays.
Serum and urine samples were immediately stored at −70 °C until testing.
The collected blood was centrifuged; serum, whole blood, and urine samples were immediately frozen and subsequently sent to Japan for analysis.
Then, a 75-g oral glucose tolerance test was performed, and samples were obtained for measurement of serum insulin and plasma glucose at 0, 30, 60, 90 and 120 min. Samples were immediately centrifuged, and serum and plasma were separated and frozen at −30 C until assayed.
Then, a 75-g oral glucose tolerance test was performed, and samples were obtained for measurement of serum insulin and plasma glucose at 0, 30, 60, 90, and 120 min. Samples were immediately centrifuged, and serum and plasma were separated and frozen at −20°C until assayed.
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