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Serum samples were frozen and stored at −20 °C until analysis by an outside laboratory, Medtox Laboratories, MN. Currently, there is no FDA guidance on establishing IVIVC for injectable dosage forms.
Serum samples were frozen and stored in liquid nitrogen for subsequent analysis.
Serum samples were frozen at -80°C.
All serum samples were frozen at −70°C until assay.
The serum samples were frozen at -20°C.
Serum samples were frozen at -70°C for future analyses.
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All serum samples were freezed and thawed once and immediately incubated at 100 °C for 10 minutes after 4 times dilution with proteomics grade water.
Following serum separation, samples were frozen until analysis.
Serum and plasma samples were frozen immediately after centrifugation and stored at −80 °C until use.
Blood was drawn after a minimum 10 h of fasting, and serum and plasma samples were frozen and stored at − 70 °C in a central repository [23].
Serum and plasma samples were frozen and stored at -80°C, and until analysis of adipokines, 5HT, tT4, cortisol, TG and cholesterol levels.
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