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Concentrations of clarithromycin in serum samples were estimated by a modification of the method described elsewhere [ 11].
Total protein, albumin, and globulin, in serum samples, were estimated with the help of AMP diagnostics company kits, while T3 and T4 were analysed by using MicroLISA kits.
The percentages of free oestradiol in serum samples were estimated from a nomogram describing the relationship between serum SHBG levels and percentages of free oestradiol measured using the centrifugal ultrafiltration-dialysis method in a reference population of normal weight pre- and postmenopausal women (Langley et al, 1985).
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The number of copies of DNA in patient serum samples was estimated by quantitative PCR (qPCR).
Glutathione (GSH) in the serum samples was estimated by the method of Moron et al. [ 19].
The lipid content in the serum samples was estimated from the total cholesterol and triglyceride concentrations (Phillips et al. 1989).
Protein concentration of samples was estimated following the Biuret method using bovine serum albumin as standard.
Protein concentration in unknown samples was estimated by comparison to a standard curve of bovine serum albumin (BSA).
Amperometric responses for blood serum samples were evaluated for standard deviation, whose average value was estimated to be ±0.3 nA (n = 20).
GCF and serum samples were collected from all subjects to estimate the levels of CSTC by ELISA.
A total of 1,190 sheep and 714 goat serum samples were collected during the 2010 survey; the estimated serologic PPRV prevalence rate was 43% (n = 1,904; 95% CI 38%47%%).
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