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Immediately after centrifugation, serum samples were divided into aliquots of 300 μl each and stored at −20 °C until use.
The serum samples were divided into aliquots and stored at −80°C until assayed.
Serum samples were divided into aliquots, and stored at -20°C.
All serum samples were divided into five groups by stratified randomization to give equal distribution of gestational ages across groups.
After centrifugation, serum samples were divided into 1 ml aliquots and immediately stored at −80°C for subsequent biochemical analyses.
Patient and control E2 serum samples were divided into low (<5 pg/ml), intermediate (5 to 10 pg/ml), and high (>10 pg/ml) levels (Table 1).
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Total blood, plasma and serum samples are divided in small aliquots and stored at −70°C.
From the mother, a set of on average 16 aliquots tubes (with a total volume of 4 ml) each from one plasma and serum sample were divided over four different microtiter trays.
To evaluate the extraction efficiency of the PAHs, a serum sample was divided into six aliquots and 27 PAHs were spiked with three of them.
Serum and urinary samples were divided into Eppendorf tubes and stored at -20°C.
Serum and TMA samples were divided into two groups at the DKK-1 median and classified as high or low DKK-1.
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