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The eluted serum samples were directly used to fill the wells of ELISA microplates (200 μL per well).
After incubation, serum samples were directly added to MCV VLP coated plates, and ELISA was performed as described above.
All serum samples were directly determined without any dilution, while the measurements of LDH, CK, αHBDH, K+, Ca, and Mg in seminal plasma were conducted after 1 : 5 dilution, and 1 : 25 dilution for GGT and P. The seminal plasma samples for the measurements of LDH, CK, αHBDH, Ca, Mg, GGT, and P were diluted with normal saline, and that for K+ was diluted with deionized water.
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All samples were directly analyzed.
After test firing, samples were directly analyzed.
Several serum samples were inactivated directly in TRIzol LS.
Serum samples were hydrolysed directly.
Questionnaires and serum samples were obtained from 38 directly exposed and 39 unexposed healthcare workers.
The serum samples were shipped on dry ice directly to the assay laboratory.
Serum samples were obtained from Bioreclamation (East Meadow, NY), which works directly with Institutional Review Boards to organize and collect specimens from disease state patients.
Serum samples were pre-diluted 1 20 and whey samples 1 2.
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