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All the serum samples were determined with electrochemiluminescence assay kit of Roche.
A total of 843 (42.15%) serum samples were determined to be positive.
It is also of note that human serum samples were determined with satisfactory results, indicating its value on clinical application.
The contents of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in serum samples were determined by a GS200 Automatic Biochemical Analyzer (Shenzhen Genius Electronics Co., Ltd., Shenzhen, China) following the instructions of the corresponding reagent kit.
Antibody titers in these serum samples were determined by haemagglutination inhibition (HI) assay as described previously [3], [4].
Antibody titers in these serum samples were determined by haemagglutination inhibition (HI) assay using a local isolated virus strain A/Guangdong Liwan/SWL1538/2009(H1N1).
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The amount of proteins in human serum samples was determined and the maximum relative error was no more than 2% and the recovery was between 95 and 105%.
The level of total tryptase (α-protryptase+β-tryptase) in serum samples was determined using a fluorescence enzyme-linked immunoassay (Unicap; Pharmacia) [11].
Immediately after collection, fasting blood samples were allowed to clot at room temperature for four hours, and the serum were collected and centrifugated at 3000 rpm/min for 15 min. Before pooling the samples, the protein concentration of the serum samples was determined by Bradford assay on a Microplate Reader (Bio-Rad, Model 680).
Stability of SAA in serum samples was determined.
The presence of human IL-1Ra in serum samples was determined by ELISA.
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