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Citrate and EDTA samples were immediately taken onto ice, and serum samples were allowed to clot at room temperature.
Serum samples were allowed to clot at room temperature, and then centrifuged and processed as described for plasma.
All samples were collected by identical means, and serum samples were allowed to clot for 60 min to ensure thorough platelet activation before centrifugation and freezing.
Serum samples were allowed to clot for 30 min at room temperature (RT), cleared by centrifugation, and stored at −20°C until analysis.
Briefly, dilutions of serum samples were allowed to interact with cyclic citrullinated peptide or TT coated in the wells of microtitre plates.
The serum samples were allowed to clot at room temperature for 30 min before centrifugation (3000 r.p.m., 4°C, 10 min) and the serum was removed and stored at −80°C until analyzed.
Similar(52)
The IL-6 in the serum sample is allowed to react immunologically with the immobilized anti-IL-6 and biotin-labeled second antibodies specific to IL-6.
Then 25 µl of 5% sodium citrate was added and incubation was continued at 56°C for 30 min. Each serum sample was allowed to cool to room temperature and 100 µl of packed chicken RBCs were added.
To obtain serum, blood samples were allowed to clot for at least 2 h at 25°C, after which they were centrifuged at 5000 g for 10 min.
The blood samples were allowed to coagulate, serum was removed after centrifugation at 1,730 × g for 20 min at 4°C and stored at -20°C prior to analysis for serum APP and the level of antibody to C. pseudotuberculosis PLD.
Blood samples were allowed to clot and serum was stored at −80°C.
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