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Serum samples were allocated to 96-well plates so that age, gender, study site (UK, Japan) and disease group (control, gastric cancer) were evenly distributed.
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Serum samples were collected from 2003 through 2007.
Liver and serum samples were collected and stored at −80 °C.
Serum samples were albumin-depleted prior to electrophoresis.
Serum samples were heat inactivated for 60 min at 56 °C and serially diluted in serum-free DMEM.
Serum samples were immediately measured for total serum bilirubin (TSB) levels using a Unistat Bilirubinometer (Reichert, Inc.).
116 dog cancer serum samples and 52 non-cancer dog serum samples were collected by Colorado State University and detailed cancer type information is in Tables S4-1.
In total, 509 human serum samples were received from various clinical laboratories.
Serum samples were collected from patients after diagnoses and before surgery.
Serum samples were obtained from whole blood after retroorbital-plexus puncture.
Serum samples were collected from Rag2KO, untreated naïve mice, and CTX-treated mice.
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