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Informed consents were obtained from all patients before the collection of patients' information and serum samples for analyses.
Informed consents were obtained from all patients before the collection of patient information and serum samples for analyses using Enzyme-linked immunosorbent assay (ELISA) to detect hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (anti-HBs), hepatitis B e antigen (HBeAg), hepatitis B e antibody (anti-HBe) and hepatitis B core antibody (anti-HBc).
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Serum samples for NGAL analyses were taken for logistical reasons in the donor hospital simultaneously with blood samples for HLA determination.
Autoantibody signatures of the protein fraction of MBD processed serum samples of control individuals were comparable to signatures derived from unprocessed serum of these individuals, indicating the suitability of MBD processed serum samples for immunological analyses upon DNA isolation.
The venous blood serum samples for the cytokine analyses were stored at -80°C until run.
Serum samples for exploratory biomarker analyses were collected at baseline, 5, 24 and 504 h (21 days) after dulanermin treatment.
Thirty-two patients had adequate serum samples for inclusion into exploratory analyses by VeriStrat.
The next examination occurred at approximately 5 years of age, before the child had received the booster vaccination; 532 cohort members participated and successfully provided a serum sample for the analyses (81%).
Hence, to examine if quantitative analysis of NGAL levels in blood could distinguish pancreatic cancer from pancreatitis, we analysed serum samples for NGAL levels.
A miscellany of further outcomes were reported mostly from retrospective laboratory analyses of serum samples for surrogate markers of disease-risk (diabetes, cardiovascular disease, maladjusted bone turnover) in subgroups of patients from other studies (mainly the RCT [ 7] and/or its extension [ 8]).
Serum samples for insulin and C-peptide were analysed by ELISA (Mercodia, Uppsala, Sweden).
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