Exact(1)
Two-fold serial dilutions of each serum sample were performed using EMEM starting with the 1 10 dilution.
Similar(59)
Glucose determination in human blood serum sample was performed using the fabricated sensing system.
With the IgG quantitiative ELISA in goats more sera samples can be assessed on each ELISA plate when compared to the conventional titre-based ELISA where end-point titrations for each serum sample are performed in rows on the plates.
Purification of IgG antibodies from this serum sample was performed as follows: the serum sample was equilibrated in a binding buffer (20 mM sodium phosphate, pH 7.0) by overnight dialysis and 3 ml of this preparation was applied to a Protein G HP affinity column (HiTrap, Amersham Biosciences, UK).
At least quadruplicate repeats of each serum samples were performed.
Harvesting serum samples were performed as previously described [ 9].
Analyses of all plasma and serum samples were performed immediately after being centrifuged.
Virus neutralization assays of heat-inactivated (30 min 56°C) ferret serum samples were performed as previously described (25 ).
Clinical chemistry evaluations of serum samples were performed using a Roche Cobas Fara chemistry analyzer (Roche Diagnostic Systems, Westwood, NJ) to numerically measure serum enzyme levels.
The preparation of protein microarray and the microarray detection of serum samples were performed according to our previous study (Li et al., 2008).
No dilution of serum samples was performed.
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