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The W29 serum sample was obtained when she received her annual checkup at the hospital in 2006.
A serum sample was obtained in all the patients at hospitalization and in 39 patients six months later.
We also recorded subsequent "dyads" of body temperature and serum phosphorus levels if available; we required that the temperature have been recorded within four hours of the time that the serum sample was obtained.
In total, for 6,385 participants from the national sample (including 645 individuals in the oversampled migrant group) and 1,518 individuals from areas with low vaccination coverage a serum sample was obtained and a questionnaire was completed.
After 6 or 12 months, treatment was discontinued since viral load did not decrease more than 2 logs and a second serum sample was obtained for analysis (T1 and T2 samples for 6 and 12 months, respectively).
A second serum sample was obtained from nine patients.
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At baseline, demographic data and a serum sample were obtained from each study subject.
Cytokine evaluation on serum samples was obtained by ELISA method with "sandwich" technique.
Plasma and serum samples were obtained from arterial blood samples taken from the indwelling arterial catheter.
Serum samples were obtained and stored at −30°C for batch testing.
Serum samples were obtained from the Saint Louis University School of Medicine (Saint Louis, MO).
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