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The CSF sample needs to be submitted together with a serum sample from the same animal and taken at the same time.
The endpoint titer is defined as the X-axis intercept of the dilution curve at an absorbance value (±three standard deviations) greater than the OD for a serum sample from a naïve mouse.
The oldest serum sample from each patient was selected for analysis.
In a typical plasma or serum sample from a healthy donor, 10 bile acids are present in amounts greater than the limit of quantitation.
Fig. 1 a 1H NMR Spectra with water suppression (Pulse sequence: p3919gp, ns = 128) blood serum sample from HC (black), and BD group (gray).
b 1H NMR Spectra recorded with T2-filter (Pulse sequence: cpmgpr1d, ns = 128) blood serum sample from HC (black), and BD group (gray).
Based on these findings, further validation studies are required based on more than 1 serum sample from different host species.
The undetectable C3 levels in the freshly collected serum sample from the C3 deficient patient also support this notion.
HCV core gene was PCR amplified from the viral cDNA after reverse transcription using the serum sample from genotype 1b HCV infected patients.
Serum samples from one healthy donor with low titer of anti-Ma2 and one serum sample from one patient with high titer of anti-Ma2.
A serum sample from a pig with acute S. japonicum infection served as a positive control, and a sample from an uninfected pig was used as a negative control.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com