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To assess which arm of the adaptive immune response was responsible for protection against challenge infection, adoptive transfer experiments were performed using serum, purified CD4+T cells and CD8+T cells obtained 4 weeks after the booster immunization with FLU-NA-DIII or FLU-NA-GFP.
Animals were bled and serum purified in polyclonal antibody preparations with concentrations of 1 μg/μL and 6 μg/μL from mice and rabbits, respectively.
Further, Gly-AGEs not only evoke oxidative and inflammatory reactions in endothelial cells (ECs) through the interaction with RAGE, but also mimic vascular permeability effects of AGE-rich serum purified from diabetic patients on hemodialysis [ 15- 20].
Further, Gly-AGEs not only evoke oxidative and inflammatory reactions in endothelial cells (ECs) through the interaction with a receptor for AGEs (RAGE), but also mimic vasopermeability effects of AGE-rich serum purified from diabetic patients on hemodialysis.
Reverse transcriptase PCR was conducted for HCV viral RNA (GenBank accession number: AB031663) from patient serum purified using a QIAmp viral RNA Mini kit (Qiagen K.K). with the PCR primers designed to the sequence of 5' non-coding region and core.
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RNA from 50 μl serum was purified using the Serum NucleoSpin miRNA kit (Macherey-Nagel, Germany) as described by the manufacturer.
In the in vitro studies, human proximal tubular epithelial cells (PTECs) were incubated with normal human serum (NHS), P-depleted serum (PDS), purified P and/or fH.
Then the serum was purified by 50 and 33% saturated ammonium sulfate, and the polyclonal antibody immunoglobulin G (IgG) was obtained.
IE were incubated on decorin, either with or without specific serum or purified IgG.
Serum was purified by centrifugation, and stored at −20°C until radioimmunoassay was performed.
The 108-day serum was purified on the peptides crosslinked to a Sepharose 4B column.
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