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Briefly, after being cultured for ∼72 hours in RPMI 1640 with 20% fetal bovine serum, lymphocytes were synchronized by the addition of thymidine for 24 hours of culture, followed by the addition of ethidium bromide and colcemid for the last 45 minutes and 25 minutes of culture, respectively.
Plasma, serum, lymphocytes, and erythrocytes were isolated and frozen at -20°C within 2 hours.
Serum, lymphocytes and stools were negative for enterovirus RNA and adenovirus DNA as detected by TaqMan based RealTime PCR methods (qualitative assays).
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This includes assessment of hematology parameters, titration of KLH-specific antibodies in serum, lymphocyte immunophenotyping in blood, thymus, spleen and lymph nodes, macroscopic pathology and histopathological evaluation of the lymphatic organs/tissues and of the injection sites.
Blood samples were collected into EDTA tubes from participants after a minimum 8-hour fast, and processed locally; serum, lymphocyte and RBC fractions were separated using a portable centrifuge and stored at −15°C.
The present study is part of the BELISS trial and included analysis of changes in histological and serum lymphocyte patterns between W0 and W28 in the 15 patients at the French centre.
As a part of the present trial, we addressed the changes in labial salivary gland (LSG) inflammation and serum lymphocyte pattern after belimumab therapy and identified predictors of response to treatment.
Detailed information about each participant is collected along with blood, serum, plasma, lymphocytes and urine samples.
Serum and lymphocytes from 23 healthcare workers were included in the present study.
Serum and lymphocytes from 23 individuals was used for the immunological assays.
Parvovirus B19 DNA as detected by TaqMan based RealTime PCR method [ 9] was positive in the serum and lymphocytes.
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