Sentence examples for serum extraction from inspiring English sources
This is important since dilution is unavoidable during serum extraction, and the performance of sensors with extracted samples is not as good as that in the buffer, as we discuss below.
To determine whether serum extraction potentially influenced the AhR agonist activity measured in the typical CALUX-like assay, we prepared serum extracts and compared the AhR agonist activities in the Faroe serum extracts and in neat sera.
Two important factors are the time the blood is left to clot prior to serum extraction and the temperature at which clotting occurs.
The bovine serum extraction procedures of total and free T3, rT3, T4 were optimised with fractional factorial designs and consisted of, respectively, deproteinisation followed by liquid liquid extraction, 30 kDA ultracentrifugation and solid phase extraction.
The dual-QDs-labeled LFS has comparative advantages in that firstly, whole blood can be detected by it without requirement of serum extraction; secondly, simultaneous quantification of CRP and PCT can be conducted within 20 min by using only 100 μL of the blood; and thirdly, its paired equipment for fluorescence detection is simple to operate and also at a low cost.
Whole blood was collected for serum extraction.
Serum extractions and cleanup were performed as described previously (Murk et al. 1997) [see Supplemental Material (doi 10.1289/ehp.0901113)].
We have previously validated the SPE-HPLC serum POP extraction method and it was demonstrated that the ER -and AR-CALUX assays respond to a wide range of different compounds including PCBs and pesticides [ 14, 15] as well as to PCB-spiked serum extracts [ 35].
Serum mRNA extraction was achieved using the QIAamp Viral RNA kit (QIAGEN, Courtaboeuf, France), as described by the manufacturer.
We have successfully utilized guanidinium thiocyanate acid phenol chloroform-based methods (i.e. TRIzol LS) [ 40] for serum RNA extraction.
In this study, we evaluated the feasibility of automating the serum RNA extraction procedure in order to increase the throughput of the assay.
