Serum concentration of immunoglobulins.
The serum concentration was calculated according to the standard curve generated.
For the vitamin D deficiency group the mean serum concentration was 10 micrograms per liter.
Maximum serum concentration (Cmax), area under the serum concentration time curve (AUC), time to maximum serum concentration (tmax) and elimination half life (t1/2) where feasible, were calculated.
To minimize cell proliferation, we used low serum concentration (3% FBS) and 2% DMSO until the end of the experiment.
The serum concentration of palmitic acid and body weight of C57BL/6 and Jα18 Knockout (KO) mice were measured.
We lesioned the adrenal glands of mice, which resulted in decreased serum concentration of corticosterone (Fig. 6a,b).
Within-day precision (COV; n = 10) is 1.2% for PGB (serum concentration 10.0 mg/l), 1.1% for GBP (serum concentration 15.8 mg/l) and 0.3% for VGB (serum concentration 15.5 mg/l).
In these circumstances, the volume of fluid in the circulation (plasma volume) is reduced and the serum concentration of solutes (osmolality) is therefore proportionately increased.
Supporting this hypothesis, we found that a single injection of FBP markedly increases serum concentration of adenosine, which was completely abrogated by inhibition of CD39 or CD73.
We used albumin at a concentration up to the one corresponding to 100% of serum concentration (0.4 mM) with an assumption of maximum disruption of BBB.
