Blood specimens for HIV testing are frequently collected using serum collection tubes.
Whole blood was collected into sterile serum collection tubes containing no anticoagulant (Becton, Dickinson & Company, Sparks, MD, USA).
After standard blood collection in a polyethylene donation bag, residual blood remaining in the tubing was collected into 3ml EDTA and serum collection vacutainers.
For serum collection, mice were anesthetized, and blood was collected by cardiac puncture.
Cattle were restrained in standing or recumbent position and 10 to 15 mL of blood was collected from the jugular vein into a serum collection tube.
At nine months, 1 ml of blood was collected into a serum separator tube (Becton-Dickinson) for serum collection from 194 infants.
Cancer patients with the following characteristics were excluded: (1) treatment with surgical operation, chemotherapy, or radiotherapy prior to serum collection, and (2) low-quality microarray data.
However, low antibody levels to MSP2 appeared to be associated with increased susceptibility to malaria within people who were parasite negative at the time of serum collection.
Formulations were administered subcutaneously to BALB/c mice at day 0 (prime) and 14 (boost) followed by serum collection on day 28.
For serum collection, retro-orbital bleeds were performed under isoflurane anesthesia on days 0, 7, 14, 21, 28, and 35, with the final bleed on day 42 or 63.
Blood samples were harvested from mouse orbital sinus by capillary tube then spun down at 12,000 rpm for serum collection.
