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Total RNA was extracted from the serum, cells, or tissues using TRIzol Reagent or Trizol LS Reagent (Invitrogen) according to the manufacturer's instructions.
For example, embedding molecular-scale computing devices in biological materials such as serum, cells, or tissues could be used to detect, and possibly treat, disease.
Upon re-addition of serum, cells synchronously progressed throughout G1 (Fig. 6F).
PC12 were cultured in DMEM media containing 6% fetal calf serum cells and 6% of horse serum.
Trypsin was inactivated with 5% fetal calf serum, cells were fixed and permeabilized simultaneously with cold methanol for 1 min then washed with PBS.
Eighteen hours after medium replacement with fresh medium containing 0.5% serum, cells were treated with recombinant human EGF 100 ng/ml (Promega Corporation, Madison, WI, USA), or NRG1 10 ng/ml (Promega Corporation) for 24 h.
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Current projects underway at the Center include research on Maternal Serum Cell-Free Fetal DNA, Citizen Science, and Criminal Justice Perceptions of Offenders with Genetic Developmental Disorders.
Conclusion: Down syndrome pregnancies exhibit 1.7-fold higher levels of maternal serum cell-free f-DNA compared with matched controls.
Single Molecule Free Solution Hydrodynamic Separation for Size Profiling of Serum Cell-Free DNA.
The comprehensive measurement of serum cell signal phosphoproteins is useful for the detection of PaCa.
Besides tissue and serum, cell lines offer good models to discover cancer biomarkers.
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